Calibration Problem in Laser Ektacytometry of Erythrocytes

The problem of measuring the deformability of erythrocytes by laser diffractometry in shear flow (ektacytometry) is considered. The question of how the shape of the isointensity line in the diffraction pattern, which arises when a laser beam is scattered by an ensemble of erythrocytes, is related to the level of light intensity on this line, is analyzed. A simplified algorithm for measuring the parameters of the distribution of erythrocytes in deformability is proposed, which does not require photometry of the central part of the diffraction pattern.