Multi spectroscopic and molecular simulation studies of propyl acridone binding to calf thymus DNA in the presence of electromagnetic force

Sharifi-Rad, Atena and Amiri-Tehranizadeh, Zeinab and Talebi, Atiye and Nosrati, Niknaz and Medalian, Morvarid and Pejhan, Mahtab and Hamzkanloo, Nazanin and Saberi, Mohammad Reza and Mokaberi, Parisa and Chamani, Jamshidkhan (2023) Multi spectroscopic and molecular simulation studies of propyl acridone binding to calf thymus DNA in the presence of electromagnetic force. BioImpacts, 13 (1). pp. 5-16. ISSN 2228-5660

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Abstract

Multi spectroscopic and molecular simulation studies of propyl acridone binding to calf thymus DNA in the presence of electromagnetic force Atena Sharifi-Rad Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran http://orcid.org/0000-0002-9300-5788 Zeinab Amiri-Tehranizadeh Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Atiye Talebi Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran Niknaz Nosrati Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran Morvarid Medalian Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran Mahtab Pejhan Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran Nazanin Hamzkanloo Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran Mohammad Reza Saberi Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Parisa Mokaberi Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran Jamshidkhan Chamani Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran http://orcid.org/0000-0003-2562-2458

Introduction: Here, the interaction behavior between propyl acridones (PA) and calf thymus DNA (ct-DNA) has been investigated to attain the features of the binding behavior of PA with ct-DNA, which includes specific binding sites, modes, and constants. Furthermore, the effects of PA on the conformation of ct-DNA seem to be quite significant for comprehending the medicine’s mechanism of action and pharmacokinetics. Methods: The project was accomplished through means of absorbance studies, fluorescence spectroscopy, circular dichroism, viscosity measurement, thermal melting, and molecular modeling techniques. Results: The intercalation of PA has been suggested by fluorescence quenching and viscosity measurements results while the thermal melting and circular dichroism studies have confirmed the thermal stabilization and conformational changes that seem to be associated with the binding. The binding constants of ct-DNA-PA complex, in the absence and presence of EMF, have been evaluated to be 6.19 × 104 M-1 and 2.95 × 104 M-1 at 298 K, respectively. In the absence of EMF, the ∆H0 and ∆S0 values that occur in the interaction process of PA with ct-DNA have been measured to be -11.81 kJ.mol-1 and 51.01 J.mol-1K-1, while in the presence of EMF they were observed to be -23.34 kJ.mol-1 and 7.49 J.mol-1K-1, respectively. These numbers indicate the involvement of multiple non-covalent interactions in the binding procedure. In a parallel study, DNA-PA interactions have been monitored by molecular dynamics simulations; their results have demonstrated DNA stability with increasing concentrations of PA, as well as calculated bindings of theoretical ΔG0. Conclusion: The complex formation between PA and ct-DNA has been investigated in the presence and absence of EMF through the multi spectroscopic techniques and MD simulation. These findings have been observed to be parallel to the results of KI and NaCl quenching studies, as well as the competitive displacement with EB and AO. According to thermodynamic parameters, electrostatic interactions stand as the main energy that binds PA to ct-DNA. Regarding the cases that involve the Tm of ct-DNA, EMF has proved to increase the stability of binding between PA and ct-DNA.
04 30 2022 01 01 2023 5 16 1 10.34172/crossmark_policy bi.tbzmed.ac.ir false Tabriz University of Medical Sciences Tabriz University of Medical Sciences 2021-01-02 2021-04-10 2022-04-30 10.34172/bi.2022.23592 20230108213046 https://bi.tbzmed.ac.ir/Article/bi-23592 https://bi.tbzmed.ac.ir/PDF/bi-13-5.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-5.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-5.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-5.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-5.pdf 10.1021/ed044pA552.2 Anticancer Agents Med Chem Acridone-based antitumor agents: a mini-review Alwan WS 15 1012 2015 10.2174/1871520615666150113104457 10.3389/fmolb.2017.00075 Reiche MA, Warner DF, Mizrahi V. Targeting DNA Replication and Repair for the Development of Novel Therapeutics against Tuberculosis. Front Mol Biosci 2017. 4: 75. https://doi.org/10.3389/fmolb.2017.00075. Bioorg Med Chem Synthesis and chemical characterization of 2-methoxy-N(10)-substituted acridones needed to reverse vinblastine resistance in multidrug resistant (MDR) cancer cells Krishnegowda G 10 2367 2002 10.1016/S0968-0896(02)00068-8 10.1007/s13738-019-01757-5 10.1016/j.aca.2013.02.005 10.1021/jf2019006 10.1371/journal.pone.0103878 10.1002/jcc.21256 10.1016/0010-4655(95)00042-E 10.1021/ja00124a002 10.1186/1756-0500-5-367 10.1021/ci500020m 10.1016/0263-7855(96)00018-5 10.1002/jcc.20110 10.1098/rspa.1924.0082 J. E. Jones DS. On the determination of molecular fields. —II. From the equation of state of a gas. Proc R Soc Lond A Math Phys Sci 1924; 106: 463. https://doi.org/10.1098/rspa.1924.0082. 10.3389/fchem.2016.00033 10.1039/C3AY42093K 10.1038/s41565-019-0620-x Doppagne B, Neuman T, Soria-Martinez R, Parra Lopez L, Bulou H, Romeo M, et al. Single-molecule tautomerization tracking through space- and time-resolved fluorescence spectroscopy. Nat Nanotechnol 2020; 15; 207–211. https://doi.org/10.1038/s41565-019-0620-x. 10.1111/php.12112 10.1016/j.jlumin.2008.02.004 V. Anbazhagan, R. Renganathan,Anbazhagan V, Renganathan R. Study on the binding of 2, 3-diazabicyclo [2.2. 2] oct-2-ene with bovine serum albumin by fluorescence spectroscopy. J Lumin 2008; 128: 1454-8. https://doi.org/10.1016/j.jlumin.2008.02.004. 10.1371/journal.pcbi.1005468 10.1016/j.tibtech.2007.08.003 10.1016/j.bbrep.2017.07.001 10.3390/toxins9070209 10.1039/C7RA05504H 10.1529/biophysj.105.059790 10.1016/j.arabjc.2014.06.001 10.1038/s42004-020-00392-z 10.1002/9781118120392.ch17 Kypr J, Kejnovska I, Bednářová K, Vorlickova M. Circular Dichroism Spectroscopy of Nucleic Acids. In: Berova N, Polavarapu LP, Nakanishi K, Woody RW. Comprehensive Chiroptical Spectroscopy : Applications in Stereochemical Analysis of Synthetic Compounds, Natural Products, and Biomolecules. Hoboken (NJ, USA): John Wiley & Sons; 2012. p. 575-586. https://doi.org/10.1002/9781118120392.ch17. 10.1021/acsnano.8b03146 Kneer M, Roller M, Lucas V, Schreiber R, Govorov A, Liedl T. Circular Dichroism of Chiral Molecules in DNA-Assembled Plasmonic Hotspots. ACS Nano 2018; 12 :9: 9110-9115. https://doi.org/10.1021/acsnano.8b03146. 10.3390/ijms13033394 Molecules Synthesis, DNA Binding and Topoisomerase I Inhibition Activity of Thiazacridine and Imidazacridine Derivatives Lafayette E 18 15035 2013 10.3390/molecules181215035

Item Type: Article
Subjects: Digital Open Archives > Medical Science
Depositing User: Unnamed user with email support@digiopenarchives.com
Date Deposited: 01 Apr 2023 06:58
Last Modified: 28 Aug 2024 13:10
URI: http://geographical.openuniversityarchive.com/id/eprint/748

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